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Reagent Storage And Stability

Store the TIBC Reagents at 2-8˚C. When stored as directed, the reagent is stable until the expiration date stated on the label.

 

Working Reagent Preparation

Catachem TIBC reagents are packaged in ready-to-use form.. No preparation is required. Upon opening, the Working Reagents are stable  for at least 30 days stored at 2-8º C and capped while not in use.

 

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.00010-0.00012 absorbance units per ug/DL.

Linear Range: In this method there is no significant nonlinearity over the range of 0-600 µg/DL.

Precision: Within-run and day-to-day precision is summarized below.

 

 

 

 

 

 

 

 

 

 

                                                                                    

Intended Use

For IN VITRO quantitative determination of Total Iron Binding Capacity (TIBC) in serum only for automated and manual applications.

 

Method Principle

In acid buffered color reagent containing Chromazurol B and excess ferric iron, transferrin bound iron from the serum sample dissociates into free ferric (fe) iron. These free iron ions and the excess iron in the reagent form a colored complex with the excess chromazurol-B and an increase in absorbance is observed. Addition of a strong ionic strength buffer, shifts the pH to the alkaline side increasing the affinity of transferrin for iron to the extend of extracting iron from the iron-dye complex until it becomes totally saturated with iron and as a result of this saturation the absorbance decreases. The decrease in absorbance is directly proportional to the TIBC. The following steps illustrate the reaction that take place in the TIBC procedure.

TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT  06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com