Correlation

A comparison of this method using an automated analyzer and a reference method based on the CO and CE reaction resulted in the following regression statistics:

Preparation Of Working Reagent

Catachem Cholesterol reagent is packaged in ready-to-use form. No preparation is required.

 

Storage and Stability

Store the Cholesterol Reagent at 2-8°C. When stored as directed, the reagent is stable until the expiration date stated on the label. When stored as directed, the Working Cholesterol Reagent is stable for 12 months at 2-8°C and 12 weeks at room temperature.

 

Method Performance Characteristics

Sensitivity: 0.0010-0.0020 absorbance units per mg/dL.

Linear Range:   0-600 mg/dL.

Precision:  Within-run and day-to-day precision is summarized below.

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Cholesterol  in serum using manual or automated applications.

 

Method Principle

Cholesterol esters present in the serum sample are hydrolyzed into free Cholesterol and fatty acids by pancreatic Cholesterol esterase (CE). The free Cholesterol formed and in the presence of oxygen, is then oxidized to cholest-4-en-3-one by Cholesterol oxidase (CO) with concomitant production of hydrogen peroxide. The hydrogen peroxide produced is quantitatively determined by coupling 4-aminoantipyrine with p-hydroxybenzoic acid where a quinoneimine dye with maximum absorption at 500nm is produced. The intensity of the dye color thus produced is directly proportional to the concentration of the total Cholesterol in the serum sample. The reaction scheme illustrates the reactions that occur in this method.

TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT  06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com