Correlation

A comparison of this method using an automated analyzer and a reference method based upon the hexokinase-G6PDH reaction resulted in the following regression statistics.

Method Performance Characteristics

Sensitivity: 0.0008 - 0.0012 absorbance units per mg/dL.

Linear Range: 0-600 mg/dL.

Precision: Within-run and day-to-day precision is summarized below.

Intended Use

For IN VITRO diagnostic use in the quantitative determination of γ-Glucose in serum using manual or automated applications.

 

Method Principle

The enzyme hexokinase catalyzes the phosphorilation of glucose in the presence of ATP and magnesium ions. The resultant glucoase-6-phosphate is then oxidized to 6-phosphoglucono lactone with concomitant reduction of nicotinamide adenine dinucleotide (NAD). The amount of NADH produced is proportional to the glucose present in the serum sample and it is quantitated at 340nm. The reaction scheme illustrates the reactions that occur in this method.

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