Correlation

A comparison of this method using an automated analyzer and a referenced procedure resulted in the following regression statistics.

Preparation Of Working Reagents

Catachem γ-GT Working Reagent is prepared by adding the required volume of distilled or deionized water as indicated on the label, to the required number of vials of γ-GT Reagent. 

 

Reagent Storage And Stability

Catachem γ-GT Reagent is stored at 2-8°C. When stored as directed, the reagent is stable until the expiration date stated on the label. The γ-GT Working Reagent is stored at 2-8°C. When prepared and stored as directed, the γ-GT  Working Reagent  is stable for ninety days.

 

Method Performance Characteristics

Sensitivity:  0.00085 - 0.0015 absorbance units/u/L.

Linear Range: 0-1000 u/L

Precision: Within-run and day-to-day precision is summarized below:

Intended Use

For IN VITRO diagnostic use in the quantitative determination of γ-Glutamyl Transpeptidase (γ-GT) in serum using manual or automated applications.

 

Method Principle

Gamma Glutamyltransferase transfers the Gamma Glutamyl group from the substrate Gamma Glutamyl-p-nitroanilide (GNA) which has maximum absorbance at 405nm. The rate of increase in absorbance is directly proportional to the GGT enzyme activity in the serum sample. The reaction scheme illustrates the reaction that occurs in this method.

TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT  06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com