Correlation

A comparison of this method using an automated analyzer and Catachem Hexokinase as the reference method, resulted in the following regression statistics.

Preparation Of Working Reagent

Catachem Glucose Oxidase Reagent is ready for use. No preparation is required.

 

Reagent Storage And Stability

Catachem Glucose Oxidase Reagent is stored at 2-8°C. When stored as directed, this reagent is stable until the expiration date stated on the label.

 

Method Performance Characteristics

Sensitivity:  0.0008 - 0.0012 absorbance units per mg/dL.

Linear Range:  0-600 mg/dL.

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Glucose in serum using manual or automated applications.

 

Method Principle

Glucose Oxidase catalyzes the oxidation of glucose to yield D-glucono-d-lactone and hydrogen peroxide. The hydrogen peroxide is quantitated via the peroxidase reaction where oxygen is transferred from hydrogen peroxide to a chromogenic acceptor producing a quinoneimine dye. The intensity of the dye color is directly proportional to the concentration of glucose in the serum sample. The reaction scheme illustrates the reactions that occur in this method:

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Phone:     203-335-9277

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Email:

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