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Correlation A comparison of the Catachem Magnesium method was carried out using an automated analyzer and against a reference method based on the calmagite reaction. The following regression statistics were observed. |
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Preparation Of Working Reagent Catachem Magnesium Reagent is packaged in a ready-to-use form. No preparation is required.
Reagent Storage And Stability Catachem Magnesium Reagent is stored at 2-8°C. When stored as directed, the reagent is stable until the expiration date stated on the label.
Method Performance Characteristics Sensitivity: 0.05-0.07 absorbance units/mg/DL. Linear Range: 0.0 - 12.0 mg/dL. Precision: Within-run and day-to-day precision is summarized below: |
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Intended Use For IN VITRO diagnostic use in the quantitative determination of Magnesium in serum using manual or automated applications.
Method Principle The serum sample is mixed with the Magnesium reagent where the total Magnesium ions react with the calmagite indicator to form a pink Magnesium-chelate complex with concomitant reduction of the reagent absorbance which has a broad absorbance peak between 600nm and 650nm. The decrease in absorbance is directly proportional to the Magnesium concentration in the serum sample. The following reaction scheme illustrates the events that take place in this Magnesium procedure. |
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TO CONTACT US:
CATACHEM, INC. 955 Connecticut Avenue Suite No. 4106 Bridgeport, CT 06607
Phone: 203-335-9277 Fax: 203-335-9977
Email: catachem@catacheminc.com
URL: www.catacheminc.com |
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Magnesium |
Within-Run Precision |
Total Precision |
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|
MEAN |
SD |
CV |
SD |
CV |
|
mg/dL |
mg/dL |
% |
mg/dL |
% |
|
1.7 |
0.04 |
2.40 |
0.13 |
7.70 |
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7.1 |
0.06 |
0.80 |
0.02 |
1.30 |
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12.2 |
0.08 |
0.70 |
0.20 |
1.80 |
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Correlation Data |
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|
Parameter |
Data Observed |
|
N |
96 |
|
Range (mg/dL) |
1.3-11.1 |
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Regression |
Y = 0.956 + 0.28 |
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Correlation |
r = 0.999 |
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Sy.x |
0.16 |