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Correlation A comparison of the Catachem Magnesium method was carried out using an automated analyzer and against a reference method based on the calmagite reaction. The following regression statistics were observed. |
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Preparation Of Working Reagent Catachem Magnesium Reagent is packaged in a ready-to-use form. No preparation is required.
Reagent Storage And Stability Catachem Magnesium Reagent is stored at 2-8°C. When stored as directed, the reagent is stable until the expiration date stated on the label.
Method Performance Characteristics Sensitivity: 0.05-0.07 absorbance units/mg/DL. Linear Range: 0.0 - 12.0 mg/dL. Precision: Within-run and day-to-day precision is summarized below: |
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Intended Use For IN VITRO diagnostic use in the quantitative determination of Magnesium in serum using manual or automated applications.
Method Principle The serum sample is mixed with the Magnesium reagent where the total Magnesium ions react with the calmagite indicator to form a pink Magnesium-chelate complex with concomitant reduction of the reagent absorbance which has a broad absorbance peak between 600nm and 650nm. The decrease in absorbance is directly proportional to the Magnesium concentration in the serum sample. The following reaction scheme illustrates the events that take place in this Magnesium procedure. |
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TO CONTACT US:
CATACHEM, INC. 353 Christian Street Suite 2 Oxford, CT 06478
Phone: 203-262-0330 Fax: 203-262-9836
Email: catachem@catacheminc.com
URL: www.catacheminc.com |
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Magnesium |
Within-Run Precision |
Total Precision |
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|
MEAN |
SD |
CV |
SD |
CV |
|
mg/dL |
mg/dL |
% |
mg/dL |
% |
|
1.7 |
0.04 |
2.40 |
0.13 |
7.70 |
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7.1 |
0.06 |
0.80 |
0.02 |
1.30 |
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12.2 |
0.08 |
0.70 |
0.20 |
1.80 |
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Correlation Data |
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|
Parameter |
Data Observed |
|
N |
96 |
|
Range (mg/dL) |
1.3-11.1 |
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Regression |
Y = 0.956 + 0.28 |
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Correlation |
r = 0.999 |
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Sy.x |
0.16 |