Correlation

A comparison of Catachem Uric Acid method using an automated analyzer and a reference Uric Acid method based upon the uricase peroxidase reaction resulted in the following regression statistics:

Preparation Of Working Reagent

Catachem Uric Acid reagent is packaged ready for use. No preparation is required.

 

Reagent Storage And Stability

Store the Catachem UA Reagent at 2-8°C. When stored as directed, this reagent is stable until the expiration date stated on the label. Once opened, keep reagent at 2-8°C and capped while not in use.

 

Method Performance Characteristics

Sensitivity: 0.014 - 0.018 absorbance units per mg/dL.

Linear Range: 0-20 mg/dL.

Precision: Within-run and day-to-day precision is summarized below.

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Uric Acid in serum using manual or automated applications.

 

Method Principle

The uricase enzyme catalyzes the oxidation of uric acid to produce allantoin and hydrogen peroxide. The hydrogen peroxide formed is quantitated by oxidative coupling of                    4-aminoantipyrine with 3-5 dichloro-2-hydroxybenzene sulfonic acid (DHBS) in the presence of peroxidase. The intensity of the color produced is directly proportional to the concentration of the uric acid in the sample. The color complex formed is read at 500nm. The reaction scheme illustrates the reactions that occur in this method.

TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT  06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com