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TO CONTACT US:
CATACHEM, INC. 955 Connecticut Avenue Suite No. 4106 Bridgeport, CT 06607
Phone: 203-335-9277 Fax: 203-335-9977
Email: catachem@catacheminc.com
URL: www.catacheminc.com |
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Correlation A comparison of this method using Catachem’s Mercuric Thiocynate Chloride method on an automated analyzer resulted in the following regression statistics. |
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Preparation Of Working Reagents Catachem Chloride 340nm reagents are packaged in ready-to-use form. No preparation is required.
Reagent Storage And Stability Store Catachem Chloride 340nm reagents at room temperature. When stored as directed, the reagents are stable until the expiration date stated on the label.
Method Performance Characteristics Sensitivity: The sensitivity of this method is 0.004 - 0.0045 absorbance units per mEq/L. Linear Range: In this method there is no significant nonlinearity over the range of 0-150 mEq/L. Precision: Within-run and day-to-day precision is summarized below. |
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Intended Use For IN VITRO diagnostic use in the quantitative determination of Chloride in serum using manual or automated applications.
Method Principle Chloride ions present in the serum sample interact with Ferric ions in dilute acid to form a yellow chloro-complex with maximum absorption at 340nm. The intensity of the color thus produced is directly proportional to the concentration of the chloride ion in the serum sample. The reaction volume illustrates the reaction that occurs in this method. |
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Chloride |
Within-Run Precision |
Total Precision |
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MEAN |
SD |
CV |
SD |
CV |
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mEq/L |
mEq/L |
% |
mEq/L |
% |
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77 |
0.83 |
1.10 |
1.07 |
1.40 |
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96 |
0.84 |
0.87 |
1.70 |
1.20 |
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120 |
1.26 |
1.40 |
1.61 |
1.30 |
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Correlation Data |
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Parameter |
Data Observed |
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N |
50 |
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Range |
90 - 110 mEq/L |
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Regression |
1.14x + 18.4 |
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Correlation |
0.987 |
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Sy.x |
1.6 |