TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT   06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com

 

                                 

 

                

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                                                                    

Correlation

A comparison of this method using Catachem’s Mercuric Thiocynate Chloride method on an automated analyzer  resulted in the following regression statistics.

Preparation Of Working Reagents

Catachem Chloride 340nm reagents are packaged in ready-to-use form. No preparation is required.

 

Reagent Storage And Stability

Store Catachem Chloride 340nm reagents at room temperature. When stored as directed, the reagents are stable until the expiration date stated on the label.

 

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.004 - 0.0045 absorbance units per mEq/L.

Linear Range: In this method there is no significant nonlinearity over the range of               0-150 mEq/L.

Precision: Within-run and day-to-day precision is summarized below.

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Chloride in serum using manual or automated applications.

 

Method Principle

Chloride ions present in the serum sample interact with Ferric ions in dilute acid to form a yellow chloro-complex with maximum absorption at 340nm. The intensity of the color thus produced is directly proportional to the concentration of the chloride ion in the serum sample. The reaction volume illustrates the reaction that occurs in this method.

Chloride

Within-Run Precision

Total Precision

MEAN

SD

CV

SD

CV

mEq/L

mEq/L

%

mEq/L

%

77

0.83

1.10

1.07

1.40

96

0.84

0.87

1.70

1.20

120

1.26

1.40

1.61

1.30

Correlation Data

Parameter

Data Observed

N

50

Range

90 - 110 mEq/L

Regression

1.14x + 18.4

Correlation

0.987

Sy.x

1.6