Correlation

A comparison of this method using an automated analyzer and a reference method resulted in the following regression statistics.

Preparation Of Working Reagent

Catachem ALP Working Reagent is prepared by combining reagent-R1 (ALP Diluent ) with the activator reagent-R2 (PNPP Substrate)

 

Reagent Storage And Stability

ALP Reagents are stored at 2-8°C. When stored as directed, the reagents are stable until the expiration date stated on the label. When prepared and stored as directed, the Working PNPP Reagent is stable for fifteen days at 2-8°C.

 

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.00060-0.00080 absorbance units per u/L.

Linear Range: In this method there is no significant nonlinearity over the range of 0-12000 u/L.

Precision: Within-run and day-to-day precision is tabulated on the tale below:

Intended Use

For IN VITRO quantitative determination of Alkaline Phosphatase in serum using manual or automated applications.

 

Method Principle

Alkaline Phosphatase catalyzes the conversion of P-nitrophenyl phosphate (PNPP) to P-nitrophenol. P-nitrophenol is a bright yellow-colored compound which has maximum absorbance at 405nm. The rate of increase in absorbance from P-nitrophenyl phosphate (colorless) to P-nitrophenol (color) is directly proportional to the AP enzyme activity in the serum sample. The reaction scheme illustrates the reaction that occurs in this method.

TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT  06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com

ALKALINE PHOSPHATASE (ALP) REAGENT