Intended Use

For IN VITRO quantitative determination of Albumin in serum or plasma using manual or automated applications.

 

Method Principle

BCG dye at a pH of 4.2 and in the presence of a non-ionic surfactant Brij-35 specifically binds albumin to form a colored BCG-Albumin complex with maximum absorbance at 630nm. The increase in absorbance is directly proportional to the Albumin concentration present in the sample.

 

                                               

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Correlation

A comparison of this method using an automated analyzer and a reference method based upon the BCG reaction resulted in the following regression statistics.

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.140-0.180 absorbance units per g/dL.

Linear Range: In this method there is no significant nonlinearity over the range of 0-6 g/dL.

Precision: Within-run and day-to-day precision is summarized below.

TO CONTACT US:

 

CATACHEM, INC.

955 Connecticut Avenue

Suite No. 4106

Bridgeport, CT  06607

 

Phone:     203-335-9277

Fax:         203-335-9977

 

Email:

catachem@catacheminc.com

 

URL:

www.catacheminc.com